Data for the Constructed Mutant: YYBGd

General Information

Name of the MutantYYBGd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size837 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybG-F: aagaagcttGCGGAAGTAACGTTACATG
Reverse Primer:(ggaggatcc tag) yybG-R: ggaggatccTTCATATGCAGAGCAGCC
Length of the Cloned Region: 7 to 225 Length: 219
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBGd