Data for the Constructed Mutant: YYBId

General Information

Name of the MutantYYBId (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size786 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybI-F: aagaagcttGTGTATGACATCAGAATG
Reverse Primer:(ggaggatcc tag) yybI-R: ggaggatccGTTTTGAAGCTGAATCCG
Length of the Cloned Region: 7 to 261 Length: 255
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBId