Data for the Constructed Mutant: YYBKd

General Information

Name of the MutantYYBKd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size753 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYBKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybK-F: aagaagcttTTCATAACTTTAGTTGCG
Reverse Primer:(ggaggatcc tag) yybK-R: ggaggatccTACTAACTTTAATTGCTC
Length of the Cloned Region: 19 to 237 Length: 219
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYBKd