Data for the Constructed Mutant: YYBLd

General Information

Name of the MutantYYBLd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size708 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybL-F: aagaagcttCTATTAATTTTATGTTTACTAC
Reverse Primer:(ggaggatcc tag) yybL-R: ggaggatccATTAATAACAGTATGTATTG
Length of the Cloned Region: 19 to 324 Length: 306
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBLd