Data for the Constructed Mutant: YYBMd

General Information

Name of the MutantYYBMd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size753 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybM-F: aagaagcttAGAAGTCATATTTATCGC
Reverse Primer:(ggaggatcc tag) yybM-R: ggaggatccAGAATCGTCAGCACATAG
Length of the Cloned Region: -753 to -753 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBMd