Data for the Constructed Mutant: YYBNd

General Information

Name of the MutantYYBNd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size435 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybN-F: aagaagcttTTCCTGAAGAGCAATTTCCG
Reverse Primer:(ggaggatcc tag) yybN-R: ggaggatccATCTGTATGAGTGGTCTG
Length of the Cloned Region: 10 to 183 Length: 174
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBNd