Data for the Constructed Mutant: YYBOd

General Information

Name of the MutantYYBOd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1305 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYBOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybO-F: aagaagcttCGCGTGAAACGGCCTGGG
Reverse Primer:(ggaggatcc tag) yybO-R: ggaggatccCATTCCAACCCTCGTGAC
Length of the Cloned Region: 34 to 282 Length: 249
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYBOd