Data for the Constructed Mutant: YYBPd

General Information

Name of the MutantYYBPd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size444 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybP-F: aagaagcttATCATTTTGACAGTACTTGCC
Reverse Primer:(ggaggactt tag) yybP-R: ggaggacttCACCTTCTCCACATAACC
Length of the Cloned Region: 7 to 126 Length: 120
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBPd