Data for the Constructed Mutant: YYBQp

General Information

Name of the MutantYYBQp (promoter fusion)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size927 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YYBQp (promoter fusion)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybQ-P3: aagaagcttAAAAGGATAGGAGCTATCATGG
Reverse Primer:(ggaggatcc tag) yybQ-R: ggaggatccTTTGATGCTTTGCTGGCG
Length of the Cloned Region: -18 to 252 Length: 271
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBQp