Data for the Constructed Mutant: YYBTd

General Information

Name of the MutantYYBTd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1977 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYBTd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybT-F: aagaagcttTATGAAAAACCGCTGTTTCGG
Reverse Primer:(ggaggactt tag) yybT-R: ggaggacttGATTCCAATCGGCATTTC
Length of the Cloned Region: 13 to 261 Length: 249
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYBTd