Data for the Constructed Mutant: YYCAd

General Information

Name of the MutantYYCAd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size2055 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYCAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yycA-F: aagaagcttACCAAAGGATTCGACGCAGCC
Reverse Primer:(ggaggatcc tag) yycA-R: ggaggatccCATGCTTTTTACAGCCGC
Length of the Cloned Region: -2055 to -2055 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYCAd