Data for the Constructed Mutant: YYCBd

General Information

Name of the MutantYYCBd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1206 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYCBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yycB-F: aagaagcttCAATCATTTTGGCTGATCACC
Reverse Primer:(ggaggatcc tag) yycB-R: ggaggatccAGGTGCAAGCGGAGAAAGAAC
Length of the Cloned Region: 28 to 207 Length: 180
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYCBd