Data for the Constructed Mutant: YYCCd
General Information
Name of the Mutant
YYCCd (disruption)
Host
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
138 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for YYCCd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
tag)
:
Reverse Primer:
(
tag)
:
Length of the Cloned Region: -1000 to -1001 Length: 2
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YYCCd