Data for the Constructed Mutant: YYCDd
General Information
Name of the Mutant
YYCDd (disruption)
Host
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
198 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for YYCDd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagaagctt
tag)
yycD-F:
aagaagcttATAAAGAAATATGCGATTACACC
Reverse Primer:
(
ggaggatcc
tag)
yycD-R:
ggaggatccCTGTTTGGCCTTTTCAATAGC
Length of the Cloned Region: 4 to 123 Length: 120
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YYCDd