Data for the Constructed Mutant: YYCDd

General Information

Name of the MutantYYCDd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size198 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYCDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yycD-F: aagaagcttATAAAGAAATATGCGATTACACC
Reverse Primer:(ggaggatcc tag) yycD-R: ggaggatccCTGTTTGGCCTTTTCAATAGC
Length of the Cloned Region: 4 to 123 Length: 120
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYCDd