Data for the Constructed Mutant: YYCEd

General Information

Name of the MutantYYCEd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size417 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYCEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yycE-F: aagaagcttTCTTTTCAAGCAGCACAAATC
Reverse Primer:(ggaggatcc tag) yycE-R: ggaggatccCAAATGATAATCGGCATGAGG
Length of the Cloned Region: 19 to 183 Length: 165
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYCEd