BSTF Data for BG11059

Family description
Mutant/Isogenic strain8G32/8G5(1), CU741c/CU741(10)
Target genescomK, comC, comE, comG, comF(2), addAB(3), nucA(4), recA(5,14), flgM(6), sacB(7), med-comZ(yjzA) (8), degR(10), rok(ykuW)(15)
cis SequenceAAAAN5TTTT(9), K-box search (18)
LigandComplex formation between ClpC, MecA and ComK inhibits ComK, and ComS dissociates this complex (11).
CommentDNA microarray analysis (16,17). Med is a positive regulator of comK (12). DegU acts as a priming protein that primes the autostimulatory transcription of comK (13). AbrB binds to the comK promoter region (19).
1. van Sinderen D, ten Berge A, Hayema BJ, Hamoen L, Venema G (1994) Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis. Mol Microbiol 11:695-703.[PMID:8196543]
2. van Sinderen D, Luttinger A, Kong L, Dubnau D, Venema G, Hamoen L (1995) comK encodes the competence transcription factor, the key regulatory protein for competence development in Bacillus subtilis. Mol Microbiol 15:455-62.[PMID:7783616]
3. Haijema BJ, Hamoen LW, Kooistra J, Venema G, van Sinderen D (1995) Expression of the ATP-dependent deoxyribonuclease of Bacillus subtilis is under competence-mediated control. Mol Microbiol 15:203-11.[PMID:7746142]
4. van Sinderen D, Kiewiet R, Venema G (1995) Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis. Mol Microbiol 15:213-23.[PMID:7746143]
5. Haijema BJ, van Sinderen D, Winterling K, Kooistra J, Venema G, Hamoen LW (1996) Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis. Mol Microbiol 22:75-85.[PMID:8899710]
6. Liu J, Zuber P (1998) A molecular switch controlling competence and motility: competence regulatory factors ComS, MecA, and ComK control sigmaD-dependent gene expression in Bacillus subtilis. J Bacteriol 180:4243-51.[PMID:9696775]
7. Msadek T, Kunst F, Rapoport G (1994) MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a pleiotropic regulator controlling competence gene expression and growth at high temperature. Proc Natl Acad Sci U S A 91:5788-92.[PMID:8016066]
8. Ogura M, Ohshiro Y, Hirao S, Tanaka T (1997) A new Bacillus subtilis gene, med, encodes a positive regulator of comK. J Bacteriol 179:6244-53.[PMID:9335269]
9. Hamoen LW, Van Werkhoven AF, Bijlsma JJ, Dubnau D, Venema G (1998) The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix. Genes Dev 12:1539-50.[PMID:9585513]
10. Ogura M, Tanaka T (1997) Bacillus subtilis ComK negatively regulates degR gene expression. Mol Gen Genet 254:157-65.[PMID:9108277]
11. Turgay K, Hamoen LW, Venema G, Dubnau D (1997) Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis. Genes Dev 11:119-28.[PMID:9000055]
12. Ogura M, Ohshiro Y, Hirao S, Tanaka T (1997) A new Bacillus subtilis gene, med, encodes a positive regulator of comK. J Bacteriol 179:6244-53.[PMID:9335269]
13. Hamoen LW, Haijema B, Bijlsma JJ, Venema G, Lovett CM (2001) The Bacillus subtilis competence transcription factor, ComK, overrides LexA-imposed transcriptional inhibition without physically displacing LexA. J Biol Chem 276:42901-7.[PMID:11555642]
14. Hamoen LW, Van Werkhoven AF, Venema G, Dubnau D (2000) The pleiotropic response regulator DegU functions as a priming protein in competence development in Bacillus subtilis. Proc Natl Acad Sci U S A 97:9246-51.[PMID:10908654]
15. Hoa TT, Tortosa P, Albano M, Dubnau D (2002) Rok (YkuW) regulates genetic competence in Bacillus subtilis by directly repressing comK. Mol Microbiol 43:15-26.[PMID:11849533]
16. Berka RM, Hahn J, Albano M, Draskovic I, Persuh M, Cui X, Sloma A, Widner W, Dubnau D (2002) Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. Mol Microbiol 43:1331-45.[PMID:11918817]
17. Ogura M, Yamaguchi H, Kobayashi K, Ogasawara N, Fujita Y, Tanaka T (2002) Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. J Bacteriol 184:2344-51.[PMID:11948146]
18. Hamoen LW, Smits WK, de Jong A, Holsappel S, Kuipers OP (2002) Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach. Nucleic Acids Res 30:5517-28.[PMID:12490720]
19. Hamoen LW, Kausche D, Marahiel MA, van Sinderen D, Venema G, Serror P (2003) The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK. FEMS Microbiol Lett 218:299-304.[PMID:12586407]
PubMed search
Ortholog SSDB     COG
Paralog SSDB
Motif SSDB
Localization PSORT   SOSUI