BSTF Data for BG10824


ORF IDBG10824
SynonymcspB
ClassHTH
FamilyCold-shock DNA-binding motif
Family description
Mutant/Isogenic strainGW1/JH642(1)
Sensor
Target genesnot
cis SequenceATTGG or CCAAT(Y-box core, single strand DNA) (2). Importance of the T base content and position within the template (7).
Ligandnot
CommentAn essential role of aromatic and basic residues in binding of single-stranded DNA containing the Y-box motif (2). Two-dimensional gel analysis (4). Extremely rapid protein folding (5). DNA macroarray analysis (6). Probable RNA chaperon (7). Localization to cytosolic spaces surrounding nucleoids (8). Complementation by translation initiation factor IF1 (9). Diffusion control in an protein folding reaction (10).
Reference
1. Willimsky G, Bang H, Fischer G, Marahiel MA (1992) Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol 174:6326-35.[PMID:1400185]
2. Graumann P, Marahiel MA (1994) The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides. FEBS Lett 338:157-60.[PMID:8307174]
3. Schroder K, Graumann P, Schnuchel A, Holak TA, Marahiel MA (1995) Mutational analysis of the putative nucleic acid-binding surface of the cold-shock domain, CspB, revealed an essential role of aromatic and basic residues in binding of single-stranded DNA containing the Y-box motif. Mol Microbiol 16:699-708.[PMID:7476164]
4. Graumann P, Schroder K, Schmid R, Marahiel MA (1996) Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol 178:4611-9.[PMID:8755892]
5. Schindler T, Schmid FX (1996) Thermodynamic properties of an extremely rapid protein folding reaction. Biochemistry 35:16833-42.[PMID:8988022]
6. Kaan T, Homuth G, Mader U, Bandow J, Schweder T (2002) Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response. Microbiology 148:3441-55.[PMID:12427936]
7. Lopez MM, Yutani K, Makhatadze GI (2001) Interactions of the cold shock protein CspB from Bacillus subtilis with single-stranded DNA. Importance of the T base content and position within the template. J Biol Chem 276:15511-8.[PMID:11278683]
8. Weber MH, Volkov AV, Fricke I, Marahiel MA, Graumann PL (2001) Localization of cold shock proteins to cytosolic spaces surrounding nucleoids in Bacillus subtilis depends on active transcription. J Bacteriol 183:6435-43.[PMID:11591689]
9. Weber MH, Beckering CL, Marahiel MA (2001) Complementation of cold shock proteins by translation initiation factor IF1 in vivo. J Bacteriol 183:7381-6.[PMID:11717297]
10. Jacob M, Schindler T, Balbach J, Schmid FX (1997) Diffusion control in an elementary protein folding reaction. Proc Natl Acad Sci U S A 94:5622-7.[PMID:9159122]
PubMed search
Ortholog SSDB     COG
Paralog SSDB
Motif SSDB
Localization PSORT   SOSUI