BSTF Data for BG10723

Family descriptionsigma54 interact motif
Mutant/Isogenic strainQB5533/168(1)
Target genesrocABC(1), rocDEF(2), rocG (4)
cis SequenceGCAAAATAATTTTGCAYT (UAS)(2). rocG expression depends on a sequence like UASlocated 1.5 kb beyond the end of the rocG coding region (4).
LigandOrnithine or citrulline is inducer (3).
CommentN-terminal part of RocR is an intramolecular repressor domain (3). AhrC involves protein-protein interaction with RocR (3). RocR-binding site as an 8 bp inverted repeat, separated by one base pair is present twice within the rocG-rocABC intergenic region, acting as both a DAS and an UAS (5).
1. Calogero S, Gardan R, Glaser P, Schweizer J, Rapoport G, Debarbouille M (1994) RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators. J Bacteriol 176:1234-41. [PMID:8113162]
2. Gardan R, Rapoport G, Debarbouille M (1995) Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol 249:843-56. [PMID:7540694]
3. Gardan R, Rapoport G, Debarbouille M (1997) Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis. Mol Microbiol 24:825-37. [PMID:9194709]
4. Belitsky BR, Sonenshein AL (1999) An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis. Proc Natl Acad Sci U S A 96:10290-5. [PMID:10468601]
5. Ali NO, Jeusset J, Larquet E, Le Cam E, Belitsky B, Sonenshein AL, Msadek T, Debarbouille M (2003) Specificity of the interaction of RocR with the rocG-rocA intergenic region in Bacillus subtilis. Microbiology 149:739-50. [PMID:12634342]
PubMed search
Ortholog SSDB     COG
Paralog SSDB
Motif SSDB
Localization PSORT   SOSUI