BSTF Data for BG10712

ClassRNA binding regulator
Family description
Mutant/Isogenic strainDB104 deltapyrR(1), BFS2815
Target genespyrR,P,B,C,AA,AB.K,D,F,E(1)
cis Sequencethree RATs (the leader, the pyrR-pyrP intercistronic region, and the pyrP-pyrB intercistronic region)(1,2).
Ligandpyrimidine in medium (1,2), UMP in cell (3). PRPP is an activator of pyr transcription (3).
CommentPyrR is a bifunctional protein: regulator and uracil phosphoribosyltransferase (1). transcriptional attenuation of the pyr operon is governed by switching between alternative antiterminator versus anti-antiterminator plus terminator structures (4). Purification and crystalization of PyrR (5,6). PyrR likely recognizes conserved RNA sequences, but only if they are properly positioned in the correct secondary structure (7). The RNA binding residues lie in two sequence segments that are conserved in PyrR proteins from many species (8).
1. Turner RJ, Lu Y, Switzer RL (1994) Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism. J Bacteriol 176:3708-22. [PMID:8206849]
2. Lu Y, Turner RJ, Switzer RL (1995) Roles of the three transcriptional attenuators of the Bacillus subtilis pyrimidine biosynthetic operon in the regulation of its expression. J Bacteriol 177:1315-25. [PMID:7868607]
3. Lu Y, Switzer RL (1996) Transcriptional attenuation of the Bacillus subtilis pyr operon by the PyrR regulatory protein and uridine nucleotides in vitro. J Bacteriol 178:7206-11. [PMID:8955403]
4. Lu Y, Turner RJ, Switzer RL (1996) Function of RNA secondary structures in transcriptional attenuation of the Bacillus subtilis pyr operon. Proc Natl Acad Sci U S A 93:14462-7. [PMID:8962074]
5. Turner RJ, Bonner ER, Grabner GK, Switzer RL (1998) Purification and characterization of Bacillus subtilis PyrR, a bifunctional pyr mRNA-binding attenuation protein/uracil phosphoribosyltransferase. J Biol Chem 273:5932-8. [PMID:9488732]
6. Tomchick DR, Turner RJ, Switzer RL, Smith JL (1998) Adaptation of an enzyme to regulatory function: structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase. Structure 6:337-50. [PMID:9551555]
7. Bonner ER, D'Elia JN, Billips BK, Switzer RL (2001) Molecular recognition of pyr mRNA by the Bacillus subtilis attenuation regulatory protein PyrR. Nucleic Acids Res 29:4851-65. [PMID:11726695]
8. Savacool HK, Switzer RL (2002) Characterization of the interaction of Bacillus subtilis PyrR with pyr mRNA by site-directed mutagenesis of the protein. J Bacteriol 184:2521-8. [PMID:11948166]
PubMed search
Ortholog SSDB     COG
Paralog SSDB
Motif SSDB
Localization PSORT   SOSUI