BSTF Data for BG10278

ClassRNA binding regulator
Family description
Mutant/Isogenic strainseveral mtrB mutants (1)
Target genestrpEDCFBA(2,3), trpG(2,3,6), yhaG(13), ycbK (11)
cis Sequence(G/U)AG repeats(3)
LigandL-Trp in medium and cell (2,3)
CommentCrystallography revealed that TRAP molecule has 11-fold symmetry and contains 11 subunits (4). eleven U/GAG repeats are bound to the surface of the protein ondecamer modified by the binding of L-tryptophan (5,7). The yczA-ycbK operon is controlled by the T-box antitermination mechanism in response to the level of uncharged tRNA(Trp) (11). Binding of TRAP to leader RNA regulates of transcription termination in the trp operon over about an 88-fold range and regulates translation of trpE over about a 13-fold range (8). NMR analysis of TRAT (9). RNA wraps around TRAP by interacting with residues Lys37, Lys56 and Arg58 (10). The 5' stem-loop functions primarily through the TRAP-dependent regulatory pathway (12). An anti-TRAP (AT) protein, encoded by yczA, conveys the metabolic signal that the cellular transfer RNA for tryptophan (tRNATrp) is predominantly uncharged (14). AT acts by competing with messenger RNA for the RNA binding domain of TRAP (15). Bound zinc is necessary for maintenance of the quaternary structure of AT (16). DNA microarray analysis (17). Translation of AT (anti-TRAP) is regulated by uncharged tRNATrp (18).
1. Gollnick P, Ishino S, Kuroda MI, Henner DJ, Yanofsky C (1990) The mtr locus is a two-gene operon required for transcription attenuation in the trp operon of Bacillus subtilis. Proc Natl Acad Sci U S A 87:8726-30. [PMID:2123343]
2. Babitzke P, Gollnick P, Yanofsky C (1992) The mtrAB operon of Bacillus subtilis encodes GTP cyclohydrolase I (MtrA), an enzyme involved in folic acid biosynthesis, and MtrB, a regulator of tryptophan biosynthesis. J Bacteriol 174:2059-64. [PMID:1551827]
3. Babitzke P, Stults JT, Shire SJ, Yanofsky C (1994) TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a multisubunit complex that appears to recognize G/UAG repeats in the trpEDCFBA and trpG transcripts. J Biol Chem 269:16597-604. [PMID:7515880]
4. Antson AA, Brzozowski AM, Dodson EJ, Dauter Z, Wilson KS, Kurecki T, Otridge J, Gollnick P (1994) 11-fold symmetry of the trp RNA-binding attenuation protein (TRAP) from Bacillus subtilis determined by X-ray analysis. J Mol Biol 244:1-5. [PMID:7525975]
5. Antson AA, Otridge J, Brzozowski AM, Dodson EJ, Dodson GG, Wilson KS, Smith TM, Yang M, Kurecki T, Gollnick P (1995) The structure of trp RNA-binding attenuation protein. Nature 374:693-700. [PMID:7715723]
6. Yang M, de Saizieu A, van Loon AP, Gollnick P (1995) Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP). J Bacteriol 177:4272-8. [PMID:7543470]
7. Babitzke P, Bear DG, Yanofsky C (1995) TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a toroid-shaped molecule that binds transcripts containing GAG or UAG repeats separated by two nucleotides. Proc Natl Acad Sci U S A 92:7916-20. [PMID:7544009]
8. Merino E, Babitzke P, Yanofsky C (1995) trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon. J Bacteriol 177:6362-70. [PMID:7592410]
9. Ramesh V, Brown T (1996) 1H-NMR characterization of L-tryptophan binding to TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis. Biochem J 315 ( Pt 3):895-900. [PMID:8645174]
10. Yang M, Chen X, Militello K, Hoffman R, Fernandez B, Baumann C, Gollnick P (1997) Alanine-scanning mutagenesis of Bacillus subtilis trp RNA-binding attenuation protein (TRAP) reveals residues involved in tryptophan binding and RNA binding. J Mol Biol 270:696-710. [PMID:9245598]
11. Sarsero JP, Merino E, Yanofsky C (2000) A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis. Proc Natl Acad Sci U S A 97:2656-61. [PMID:10706627]
12. Du H, Yakhnin AV, Dharmaraj S, Babitzke P (2000) trp RNA-binding attenuation protein-5' stem-loop RNA interaction is required for proper transcription attenuation control of the Bacillus subtilis trpEDCFBA operon. J Bacteriol 182:1819-27. [PMID:10714985]
13. Sarsero JP, Merino E, Yanofsky C (2000) A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be involved in tryptophan transport. J Bacteriol 182:2329-31. [PMID:10735881]
14. Valbuzzi A, Yanofsky C (2001) Inhibition of the B. subtilis regulatory protein TRAP by the TRAP-inhibitory protein, AT. Science 293:2057-9. [PMID:11557884]
15. Valbuzzi A, Gollnick P, Babitzke P, Yanofsky C (2002) The anti-trp RNA-binding attenuation protein (Anti-TRAP), AT, recognizes the tryptophan-activated RNA binding domain of the TRAP regulatory protein. J Biol Chem 277:10608-13. [PMID:11786553]
16. Valbuzzi A, Yanofsky C (2002) Zinc is required for assembly and function of the anti-trp RNA-binding attenuation protein, AT. J Biol Chem 277:48574-8. [PMID:12386162]
17. Berka RM, Cui X, Yanofsky C (2003) Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis. Proc Natl Acad Sci U S A 100:5682-7. [PMID:12719520]
18. Chen G, Yanofsky C (2003) Tandem transcription and translation regulatory sensing of uncharged tryptophan tRNA. Science 301:211-3. [PMID:12855807]
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Ortholog SSDB     COG
Paralog SSDB
Motif SSDB
Localization PSORT   SOSUI