Mutant list 14 (page 14)



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Gene Name Size Mutant Contributor Essential for growth on LB Product Reference
ID SubtiList BSORF This work Previous work Prediction Prophage
BG11151 yxjB yxjB 282 YXJBd Dr. Fujita No similar to rRNA methyltransferase
BG11150 yxjA yxjA 397 YXJAd Dr. Fujita No similar to pyrimidine nucleoside transport protein
BG11149 yxiT yxiT 80 YXITd Dr. Fujita No function unknown and unique
BG11148 yxiS yxiS 96 YXISd Dr. Fujita No function unknown and unique
BG11102 katE katB 686 KATBd Dr. Fujita No catalase 2
BG11146 citH citH 426 YXIQd Dr. Fujita No divalent metal ions/citrate complex secondary transporter
BG10476 bglS bglS 242 No endo-beta-1,3-1,4 glucanase Microbiol 1995 141:281-90
BG10474 licT licT 277 No transcriptional antiterminator required for substrate-dependent induction and catabolite repression of bglPH  J Bacteriol 1996 Apr;178(7):1971-9
BG11145 yxiP yxiP 226 YXIPd Dr. Fujita No function unknown and unique
BG11144 yxiO yxiO 428 YXIOd Dr. Fujita No probable transporter
BG11143 deaD deaD 479 DEADd Dr. Fujita No ATP-dependent RNA helicase
BG11142 yxiM yxiM 382 YXIMd Dr. Fujita No similar genes in B. subtilis
BG11141 yxiL yxiL 66 YXILc Dr. Ogasawara No function unknown and unique
BG11140 yxiK yxiK 153 YXIKd Dr. Fujita No function unknown and unique
BG11139 yxiJ yxiJ 98 YXIJd Dr. Fujita No function unknown and unique
BG11138 yxiI yxiI 162 YXIId Dr. Fujita No function unknown and unique
BG14170 yxzG yxzG 150 YZXGd Dr. Ogasawara No function unknown and unique
BG11137 yxiH yxiH 109 YXIHd Dr. Fujita No function unknown and unique
BG11136 yxiG yxiG 138 YXIGd Dr. Fujita No function unknown and unique
BG14167 yxzC yxzC 122 YXZCd Dr. Fujita No function unknown and unique
BG11135 yxiF yxiF 156 YXIFd Dr. Fujita No function unknown and unique
BG10798 yxxG yxxG 142 YXXGp Dr. Fujita No function unknown and unique
BG10797 wapA wapA 2334 No cell wall-associated protein Mol Microbiol 1993 Apr;8(2):299-310
BG10796 yxxF yxxF 311 YXXFd Dr. Fujita No conserved membrane protein
BG11134 yxiE yxiE 148 YXIEd Dr. Fujita No conserved protein
BG10935 bglH bglH 469 No beta-glucosidase
BG10934 bglP bglP 609 No PTS beta-glucoside-specific enzyme IIBCA component J Bacteriol 1995 Mar;177(6):1527-35
BG11071 yxxE yxxE 102 YXXEd Dr. Fujita No function unknown and unique
BG11070 yxxD yxxD 147 YXXDp Dr. Fujita No function unknown and unique
BG11133 yxiD yxiD 569 YXIDd Dr. Fujita No function unknown and unique
BG11132 yxiC yxiC 89 YXICd Dr. Fujita No similar to B. subtilis YwqI
BG11131 yxiB yxiB 121 YXIBd Dr. Fujita No similar to B. subtilis YwqH
BG11130 yxiA yxiA 469 YXIAd Dr. Fujita No similar to arabinan endo-1,5-alpha-L-arabinosidase
BG10666 hutP hutP 151 No transcriptional activator of the histidine utilization operon J Bacteriol 1988 170:3199-205
BG10667 hutH hutH 508 No histidine ammonia-lyase (histidase) J Bacteriol 1988 170:3199-205
BG10668 hutU hutU 552 No urocanate hydratase (urocanase) J Biol Chem 1970 245:3545-3548
BG11100 hutI hutI 421 No imidazolonepropionase (imidazolone-5-propionate hydrolase) J Biol Chem 1970 245:3545-3548
BG11099 hutG hutG 319 No formiminoglutamase (formiminoglutamate hydrolase) J Biol Chem 1970 245:3545-3548
BG11101 hutM hutM 475 HUTMd Dr. Fujita No histidine permease
BG10985 pdp pdp 433 No pyrimidine-nucleoside phosphorylase J Bacteriol 1996 178:424-34
BG10984 nupC nupC 393 No pyrimidine nucleoside transport protein J Bacteriol 1996 178:424-34
BG10983 dra dra 211 No deoxyribose-phosphate aldolase J Bacteriol 1996 178:424-34
BG10982 deoR deoR 313 No transcriptional repressor of the dra-nupC-pdp operon J Bacteriol 1996 178:424-34
BG10981 yxxB yxxB 289 YXXBd Dr. Fujita No conserved membrane protein
BG11894 yxeR yxeR 370 YXERd Dr. Fujita No similar to ethanolamine transporter
BG11893 yxeQ yxeQ 445 YXEQd Dr. Fujita No conserved protein
BG11892 yxeP yxeP 380 YXEPd Dr. Fujita No similar to aminoacylase
BG11891 yxeO yxeO 249 YXEOd Dr. Fujita No polar amino acid transport system ATP-binding protein
BG11890 yxeN yxeN 224 YXENd Dr. Fujita No polar amino acid transport system permease protein
BG11889 yxeM yxeM 264 YXEMp Dr. Fujita No polar amino acid transport system substrate-binding protein
BG11888 yxeL yxeL 165 YXELd Dr. Fujita No probable acetyltransferase
BG11887 yxeK yxeK 441 YXEKd Dr. Fujita No similar to monooxygenase
BG11886 yxeJ yxeJ 75 YXEJc Dr. Ogasawara No function unknown and unique
BG11885 yxeI yxeI 328 YXEId Dr. Fujita No putative penicillin amidase
BG11884 yxeH yxeH 270 YXEHd Dr. Fujita No putative hydrolase
BG11883 yxeG yxeG 185 YXEGd Dr. Fujita No function unknown and unique
BG11882 yxeF yxeF 144 YXEFd Dr. Fujita No function unknown and unique
BG11881 yxeE yxeE 121 YXEEd Dr. Fujita No spore coat protein
BG11880 yxeD yxeD 117 YXEDd Dr. Fujita No function unknown and unique
BG11879 yxeC yxeC 132 YXECd Dr. Fujita No function unknown and unique
BG11878 yxeB yxeB 319 YXEBd Dr. Fujita No putative ferrichrome transport system substrate-binding protein
BG11877 yxeA yxeA 115 YXEAd Dr. Fujita No conserved protein
BG11129 yxdM yxdM 622 YXDMd Dr. Fujita No ABC transport system permease protein
BG11128 yxdL yxdL 257 YXDLd Dr. Fujita No ABC transport system ATP-binding protein
BG11127 yxdK yxdK 325 YXDKd Dr. Fujita No two-component sensor histidine kinase. potential cognate response regulator is YxdJ
BG11126 yxdJ yxdJ 229 YXDJd Dr. Fujita No two-component response regulator. potential cognate sensor kinase is YxdK
BG11125 fbaB iolJ 290 IOLJd Dr. Fujita No fructose-bisphosphate aldolase
BG11124 iolI iolI 278 IOLId Dr. Fujita No iolI protein
BG11123 iolH iolH 289 IOLHd Dr. Fujita No iolH protein
BG10669 idh iolG 344 IOLGd Dr. Fujita No myo-inositol 2-dehydrogenase
BG11122 iolF iolF 439 IOLFd Dr. Fujita No inositol transport protein. myo-inositol catabolism
BG11121 iolE iolE 297 IOLEd Dr. Fujita No iolE protein
BG11120 iolD iolD 580 IOLDd Dr. Fujita No probable malonic semialdehyde oxidative decarboxylase
BG11119 iolC iolC 325 IOLCd Dr. Fujita No iolC protein
BG11118 iolB iolB 271 IOLBd Dr. Fujita No iolB protein
BG11117 mmsA iolA 487 IOLAd Dr. Fujita No methylmalonate-semialdehyde dehydrogenase
BG11364 iolR iolR 251 No transcriptional repressor of the myo-inositol catabolism operon (DeoR family)  J Bacteriol 1997 Jul;179(14):4591-8
BG11363 iolS iolS 310 No iolS protein  J Bacteriol 1997 Jul;179(14):4591-8
BG11362 yxcE yxcE 216 YXCEd Dr. Fujita No function unknown and unique
BG11361 yxcD yxcD 102 YXCDd Dr. Fujita No function unknown and unique
BG11360 csbC csbC 461 YXCCd Dr. Fujita No sigmaB dependent, probable sugar transport protein
BG11359 htpG htpG 626 No class III heat-shock protein (chaperone) Mol Gen Genet 1999 Apr;261(3):582-8
BG11358 yxcA yxcA 83 YXCAd Dr. Fujita No function unknown and unique
BG11357 yxbG yxbG 273 YXBGd Dr. Fujita No similar to glucose 1-dehydrogenase
BG11356 yxbF yxbF 380 YXBFd Dr. Fujita No possible transcriptional regulator (TetR family)
BG11355 aldX aldX 445 ALDXd Dr. Fujita No aldehyde dehydrogenase
BG11354 yxbD yxbD 159 YXBDd Dr. Fujita No probable acetyltransferase
BG11353 yxbC yxbC 330 YXBCd Dr. Fujita No function unknown and unique
BG11352 yxbB yxbB 244 YXBBd Dr. Fujita No putative methyltransferase
BG11351 yxbA yxbA 89 YXBAd Dr. Fujita No function unknown and unique
BG14166 yxnB yxnB 160 YXNBd Dr. Fujita No function unknown and unique
BG11116 asnH asnH 747 ASNHd/BFS54/55 Dr. Fujita/Dr. Ehrlich No asparagine synthetase (glutamine-hydrolyzing)
BG11115 yxaM yxaM 399 YXAMd Dr. Fujita No similar to antibiotic resistance protein
BG11114 yxaL yxaL 410 YXALd Dr. Fujita No similar to serine/threonine protein kinase
BG11112 yxaJ yxaJ 142 YXAJd Dr. Fujita No function unknown and unique
BG11111 yxaI yxaI 151 YXAId Dr. Fujita No conserved membrane protein
BG11110 yxaH yxaH 402 YXAHd Dr. Fujita No conserved protein
BG11109 yxaG yxaG 337 YXAGd Dr. Fujita No function unknown and unique
BG11108 yxaF yxaF 191 YXAFd Dr. Fujita No probable transcriptional regulator (TetR family)
BG14165 yxnA yxnA 356 YXNAd Dr. Fujita No similar to glucose 1-dehydrogenase
BG11106 yxaD yxaD 143 YXADd Dr. Fujita No probable transcriptional regulator (MarR family)
BG11105 yxaC yxaC 355 YXACd Dr. Fujita No peptidoglycan hydrolase, LrgB autolysin family
BG11104 yxaB yxaB 343 YXABd Dr. Fujita No general stress protein 30, similar to polysaccharide polymerization protein
BG11103 yxaA yxaA 382 YXAAd Dr. Fujita No probable glycerate kinase
BG10648 gntR gntR 243 No transcriptional repressor of the gluconate operon (GntR family) Proc Natl Acad Sci USA Jul;84_4524-8
BG10649 gntK gntK 513 No gluconokinase (gluconate kinase) J Bacteriol  1989  171:1751-1754
BG10650 gntP gntP 448 No gluconate permease J Bacteriol  1989  171:1751-1754
BG10651 gntZ gntZ 468 GNTZd Dr. Fujita No 6-phosphogluconate dehydrogenase
BG11385 ahpC ahpC 187 AHPCd Dr. Ogasawara No alkyl hydroperoxide reductase small subunit J Bacteriol 1996 178:6571-8
BG11204 ahpF ahpF 509 No alkyl hydroperoxide reductase large subunit
BG11203 bglA bglA 479 No 6-phospho-beta-glucosidase Gene 1994 140:85-90
BG14172 yyzE yyzE 76 YYZEc Dr. Ogasawara No putative PTS glucose-specific enzyme IIA component
BG11484 yydK yydK 236 YYDKd Dr. Ogasawara No negative regulator of bglA (GntR family)
BG11483 yydJ yydJ 240 YYDJd Dr. Ogasawara No conserved membrane protein
BG11482 yydI yydI 209 YYDId Dr. Ogasawara No similar to ABC transporter (ATP-binding protein)
BG11481 yydH yydH 252 YYDHd Dr. Ogasawara No membrane-embedded metalloprotease
BG11480 yydG yydG 319 YYDGd Dr. Ogasawara No conserved protein
BG11479 yydF yydF 49 YYDFc Dr. Ogasawara No function unknown and unique
BG11478 fbp fbp 671 FBPd Dr. Ogasawara No fructose-1,6-bisphosphatase
BG11477 yydD yydD 586 YYDDd Dr. Ogasawara No conserved protein
BG11476 yydC yydC 132 YYDCd Dr. Ogasawara No function unknown and unique
BG11475 yydB yydB 481 YYDBd Dr. Ogasawara No function unknown and unique
BG11474 yydA yydA 159 YYDAd Dr. Ogasawara No conserved protein
BG11472 yycS yycS 137 YYCSd Dr. Ogasawara No function unknown and unique
BG11471 yycR yycR 408 YYCRd Dr. Ogasawara No similar to formaldehyde dehydrogenase
BG11470 yycQ yycQ 82 YYCQd Dr. Ogasawara No function unknown and unique
BG11469 yycP yycP 387 YYCPd Dr. Ogasawara No conserved protein
BG11468 yycO yycO 245 YYCOd Dr. Ogasawara No function unknown and unique
BG11467 yycN yycN 156 YYCNd Dr. Ogasawara No probable acetyltransferase
BG11466 rapG rapG 365 RAPGd Dr. Ogasawara No response regulator aspartate phosphatase
BG11465 phrG phrG 38 PHRId Dr. Ogasawara No regulator of the activity of phosphatase RapG
BG10932 rocF rocF 296 No arginase  J Mol Biol 1995 Jun 23;249(5):843-56
BG10933 rocE rocE 467 No amino acid permease  J Mol Biol 1995 Jun 23;249(5):843-56
BG10722 rocD rocD 401 No ornithine aminotransferase (ornithine--oxo-acid aminotransferase)  J Mol Biol 1995 Jun 23;249(5):843-56
BG10723 rocR rocR 461 No transcriptional activator of arginine utilization operons (NtrC family)  J Bacteriol 1994 Mar;176(5):1234-41
BG11054 yyxA yyxA 400 YYXAd Dr. Ogasawara No similar to serine protease Do
BG11464 yycJ yycJ 268 YYCJd Dr. Ogasawara No conserved protein
BG11463 yycI yycI 280 YYCId Dr. Ogasawara No conserved protein
BG11462 yycH yycH 455 YYCHd Dr. Ogasawara No conserved protein
BG11461 yycG yycG 611 YYCGp Dr. Ogasawara Yes two-component sensor histidine kinase. cognate response regulator is YycF
BG10001 yycF yycF 235 YYCFp Dr. Ogasawara Yes two-component response regulator. cognate sensor kinase is YycG
BG10002 purA purA 430 PURAd Dr. Ogasawara No adenylosuccinate synthetase (IMP--aspartate ligase)
BG10003 yycE yycE 139 YYCEd Dr. Ogasawara No function unknown and unique
BG10004 dnaC dnaC 454 Yes replicative DNA helicase Microbiology 1995 141:641-4
BG10005 yycD yycD 66 YYCDd Dr. Ogasawara No function unknown and unique
BG14171 yyzB yyzB 67 YYZBc Dr. Ogasawara No function unknown and unique
BG10006 yycC yycC 46 YYCCd Dr. Ogasawara No function unknown and unique
BG10007 yycB yycB 402 YYCBd Dr. Ogasawara No probable transporter
BG10008 yycA yycA 685 YYCAd Dr. Ogasawara No conserved membrane protein
BG10009 rplI rplI 149 Yes 50S ribosomal protein L9
BG10010 yybT yybT 659 YYBTd Dr. Ogasawara No conserved membrane protein
BG10011 yybS yybS 309 YYBSd Dr. Ogasawara No conserved protein
BG10012 cotF cotF 160 No spore coat protein  J Bacteriol 1991 May;173(9):2915-9
BG10013 yybR yybR 125 YYBRd Dr. Ogasawara No conserved protein
BG10014 ppaC ppaC 309 YYBQp Dr. Ogasawara Yes inorganic pyrophosphatase
BG10015 yybP yybP 148 YYBPd Dr. Ogasawara No function unknown and unique
BG10016 yybO yybO 435 YYBOd Dr. Ogasawara No hexuronate transporter
BG10017 yybN yybN 145 YYBNd Dr. Ogasawara No function unknown and unique
BG10018 yybM yybM 251 YYBMd Dr. Ogasawara No function unknown and unique
BG10019 yybL yybL 236 YYBLd Dr. Ogasawara No similar to ABC transporter (permease)
BG10020 yybK yybK 251 YYBKd Dr. Ogasawara No function unknown and unique
BG10021 yybJ yybJ 218 YYBJd Dr. Ogasawara No hypothetical ABC transporter ATP-binding protein
BG10022 yybI yybI 262 YYBId Dr. Ogasawara No conserved protein
BG10023 yybH yybH 129 YYBHd Dr. Ogasawara No conserved protein
BG10024 yybG yybG 279 YYBGd Dr. Ogasawara No conserved protein
BG10025 yybF yybF 404 YYBFd Dr. Ogasawara No similar to antibiotic resistance protein
BG10026 yybE yybE 278 YYBEd Dr. Ogasawara No positive Regulator of yybF (LysR family)
BG10027 yybD yybD 147 YYBDd Dr. Ogasawara No probable acetyltransferase
BG10028 yybC yybC 159 YYBCd Dr. Ogasawara No conserved membrane protein
BG10029 yybB yybB 220 YYBBd Dr. Ogasawara No conserved protein
BG10030 yybA yybA 150 YYBAd Dr. Ogasawara No negative Regulator of yyaTS (MarR family)
BG10031 yyaT yyaT 148 YYATd Dr. Ogasawara No probable acetyltransferase
BG10032 yyaS yyaS 201 YYASd Dr. Ogasawara No conserved membrane protein
BG10033 yyaR yyaR 173 YYARd Dr. Ogasawara No similar to streptothricine acetyl-transferase
BG10034 yyaQ yyaQ 118 YYAQd Dr. Ogasawara No conserved protein
BG10035 yyaP yyaP 188 YYAPd Dr. Ogasawara No similar genes in B. subtilis
BG10036 tetB tetB 458 No tetracycline resistance protein J Biol Chem. 1994 Nov 4;269(44):27365-7
BG11051 tetL tetL 20 No tetracycline resistance protein leader peptide J Biol Chem 1994 269:27365-71
BG10037 yyaO yyaO 79 YYAOdd Dr. Ogasawara No conserved protein
BG10038 yyaN yyaN 138 YYANd Dr. Ogasawara No probable transcriptional regulator (MerR family)
BG10039 yyaM yyaM 305 YYAMd Dr. Ogasawara No conserved membrane protein
BG10040 yyaL yyaL 689 YYALd Dr. Ogasawara No conserved protein
BG10041 yyaK yyaK 299 YYAKd Dr. Ogasawara No putative membrane-bound metalloprotease
BG10042 yyaJ yyaJ 451 YYAJd Dr. Ogasawara No probable transporter
BG10043 maa maa 184 YYAId Dr. Ogasawara No probable maltose o-acetyltransferase
BG10044 yyaH yyaH 126 YYAHd Dr. Ogasawara No similar to lactoylglutathione lyase
BG10045 ccpB ccpB 311 CCPBd Dr. Ogasawara No transcriptional regulator involved in catabolite repression (LacI family)
BG10046 exoA exoA 252 EXOAd Dr. Ogasawara No exodeoxyribonuclease
BG10047 rpsR rpsR 79 Yes 30S ribosomal protein S18
BG10048 ssb ssb 172 Yes single-strand binding protein
BG10049 rpsF rpsF 95 Yes 30S ribosomal protein S6
BG10050 yyaF yyaF 366 YYAFd Dr. Ogasawara No GTP-binding protein
BG10051 yyaE yyaE 667 YYAEd Dr. Ogasawara No similar to formate dehydrogenase
BG10052 yyaD yyaD 338 YYADd Dr. Ogasawara No conserved membrane protein
BG10053 yyaC yyaC 205 YYACd Dr. Ogasawara No conserved protein
BG10054 spo0J spo0J 282 No stage 0 sporulation protein J  J Bacteriol 1994 Sep;176(17):5320-9
BG10055 soj soj 253 No soj protein  J Bacteriol 1994 Sep;176(17):5320-9
BG10056 yyaB yyaB 146 YYABd Dr. Ogasawara No conserved membrane protein
BG10057 yyaA yyaA 283 YYAAd Dr. Ogasawara No similar to DNA-binding protein Spo0J
BG10058 gidB gidB 239 GIDBd Dr. Ogasawara No glucose inhibited division protein B
BG10059 gidA gidA 628 GIDAd Dr. Ogasawara No glucose inhibited division protein A
BG10060 thdF thdF 459 THDFd Dr. Ogasawara No probable tRNA modification GTPase
BG10061 jag jag 208 JAG Dr. Ogasawara No SpoIIIJ-associated protein
BG10062 spoIIIJ spoIIIJ 261 No translocase for membrane proteins, essential for sigma-G activity at stage III J Gen Microbiol 1992 Dec;138 ( Pt 12):2609-18
BG10063 rnpA rnpA 116 Yes ribonuclease P protein component
BG10064 rpmH rpmH 44 Yes 50S ribosomal protein L34